Patent No. 5,221,643
Use Of Bacterial Luciferase Structural Genes For Cloning And Monitoring Gene Expression In Microorganisms And For Tagging And Identification Of Genetically Engineered Organisms
Issued: June 22, 1993
Inventor: Roman Legocki, Misuk Legocki, Aladar Szalay, all of Ithaca, NY; Thomas Baldwin, Bryan TX
A host microorganism is genetically and stably modified by the insertion into any of its non-essential chromosomal locations of a non- homologous, recombinant foreign DNA fragment, maintaining a lux AB gene of a selected bioluminescent bacterium, such as V. Harveyi such that the expression of the lux AB gene causes the production of a luciferase enzyme which, in turn, catalyzes a light-emitting reaction in the presence of aldehyde. Both Gram-positive and Gram-negative bacteria are microorganisms which may be modified in this manner. X-ray film can be used to quantify the light being emitted from a microorganism through the use of plural droplets containing the same microorganism, each with a known and related cell (or plasmid) count.
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