ESTABLISHMENT OF NEW CELL LINES FROM PSEUDALETIA UNIPUNCTA WITH DIFFERENTIAL RESPONSES TO BACULOVIRUS

Patent No. 5,686,305

Issued: November 11, 1997

Inventor: Ping Wang and Robert R. Granados, Ithaca, NY

Assignee: Boyce Thompson Institute for Plant Research, Inc., Ithaca, NY
Contact: Christopher Michaels, at Brown & Michaels, Ithaca (see below for address and phone number) - michaels@bpmlegal.com


The cell line of this patent is an ideal candidate host cell systems to study the specific interaction between the prototype baculovirus, AcMNPV, and insect cells. In addition, BTI-Pu527A7S, is a suspension cell line that produces over 100 AcMNPV OBs per cell in a stationary flask, demonstrating its superior productivity. Therefore, once this cell line is optimized for growth and infection, it has the potential to be grown in suspension culture at higher cell densities, and possibly produce even higher yields of AcMNPV OBs and recombinant proteins.


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