Baculoviruses with enhanced virion production and a method for the production of baculoviruses

Patent No.  8,703,469

Issued: April 22, 2014
Filed: September 13, 2011

Inventors: McCray; Paul (Iowa City, IA), Sinn; Patrick (Iowa City, IA), Blissard; Gary (Ithaca, NY)
Assignee: Boyce Thompson Institute for Plant Research (Ithaca, NY), University of Iowa Research Foundation (Iowa City, IA)


A method of co-expressing a portion of the VSV G protein gene or a truncated "stem" portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.

Reference to Related Applications

This application is a continuation of co-pending PCT application number PCT/US2010/026922, filed Mar. 11, 2010, entitled "BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES", which claims one or more inventions which were disclosed in Provisional Application No. 61/159,940, filed Mar. 13, 2009, entitled "BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES". The benefit under 35 USC 119(e) of the United States provisional application is hereby claimed, and the aforementioned applications are hereby incorporated herein by reference.

This application is a continuation-in-part of co-pending application Ser. No. 12/667,956, filed Jan. 6, 2010, entitled "BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES", which is a 371 national stage filing of PCT/US08/69132, filed Jul. 3, 2008, entitled "BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES", which claims one or more inventions which were disclosed in Provisional Application No. 60/948,214, filed Jul. 6, 2007, entitled "BACULOVIRUSES WITH ENHANCED VIRION PRODUCTION AND A METHOD FOR THE PRODUCTION OF BACULOVIRUSES". The benefit under 35 USC 119(e) of the United States provisional application is hereby claimed, and the aforementioned applications are hereby incorporated herein by reference.


Go Back for more representative patents.


Home || Intellectual Property || Patents || Trademarks || Copyrights


Brown & Michaels, PC
400 M & T Bank Building
118 North Tioga Street - The Commons
Ithaca, NY 14850
Phone: (607) 256-2000 Fax: (607) 256-3628

e-mail: bpm@bpmlegal.com

© 2014 Brown & Michaels PC