Improved insect cell lines, two of which are designated H5CL-B and H5CL-F (both of which are derived from the parental cell line BTI-TN-5B1-4, ATCC CRL 10859), possess the properties of increased production of baculovirus particles, increased expression of foreign proteins using a baculovirus expression system, and increased resistance to cell culture stress, relative to the parental cell line.

REFERENCE TO RELATED APPLICATIONS

This application claims an invention which was disclosed in Provisional Application No. 60/426,535, filed Nov. 15, 2002, entitled "IMPROVED CLONAL CELL LINES DERIVED FROM BTI-TN-5B1-4". The benefit under 35 USC .sctn. 119(e) of the United States provisional application is hereby claimed, and the aforementioned application is hereby incorporated herein by reference in its entirety.

For more cell line patents from Boyce Thompson Institute, see:

• 6,965,059 Method of protecting plants by introducing a gene coded for a protein which enhances virus infection of host insects - a patent on plant which produces an "enhancin" derived from an insect

• 6,858,205 GP64-null baculoviruses pseudotyped with heterologous envelope proteins for gene therapy - from Boyce Thompson Institute for Plant Reseach

• 6,770,799 Expression of recombinant human acetylcholinesterase in transgenic plants - a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase

• 6,765,127 Invertebrate intestinal mucin cDNA and related products and methods - an insect intestinal mucin protein derived from Tricoplusia ni, useful for production of transgenic cells having insecticidal activity

• 6,607,912 GP64-null baculoviruses pseudotyped with heterologous envelope proteins - baculoviruses which can be used to efficiently deliver genes to mammalian cells or organisms
• 6,472,175 and 6,261,805 Sialyiation of N-linked glycoproteins in the baculovirus expression vector system - from Boyce-Thompson Institute for Plant Research
• 6,187,558 Invertebrate Intestinal Mucin cDNA and Related Products and Methods - for enhancing the effectiveness of bioengineered pesticides and defenses of transgenic plants

• 5,962,765 Molecular cloning of a complimentary DNA sequence encoding a cuticle degrading protease produced by entomopathogenic fungi .

• 5,750,383 Baculovirus Cloning System - a marker-rescue system using an essential gene

• 5,717,069 DNA Sequence Coding for Enhancin Polypeptide which Enhances Virus Infection of Host Insects - a development of the Boyce Thompson Institute which enhances the absorbtion of virus particles by the midgut cells of insects, increasing mortality in response to biopesticides - from Boyce-Thompson Institute for Plant Research.
• 5,686,305 Establishment Of New Cell Lines From Pseudaletia Unipuncta With Differential Responses To Baculovirus - a new insect cell line with superior production of baculoviruses.

• 5,593,669 and 6,090,379 Stable Pre-Occluded Virus Particle - A method of infecting insects using a baculovirus, including use as an insecticide. 

• 5,300,435 Trichoplusia ni Cell Line which Supports Replication of Baculoviruses - "High five(tm) cells" - A cell line derived from the eggs of the Cabbage Looper, which, combined with genetically engineered viruses, shows promise to be one of the best producers of biopesticides, vaccines, etc. on the market. .
• 5,298,418 Cell Line from Midgut Tissue of T.Ni - an insect cell line derived from the Cabbage Looper
• 5,221,623 Use of Bacterial Luciferase Genes for Cloning and Monitoring Gene Expression in Microorganisms and for Tagging and Identification of Genetically Engineered Organisms The same mechanism which makes fireflies glow is used as a genetic tag to allow scientists to monitor the results of genetic engineering. (Also patented in Canada and Europe)

Go Back for more representative patents.

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