Inventors: Mor; Tsafrir S. (Department of Plant Biology, Room 637, Arizona State University, P.O. Box, Tempe, AZ 85287-1601); Soreq; Hermona (Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, IL 91904); Arntzen; Charles J. (Department of Plant Biology, Room 637, Arizona State University, P.O. Box, Tempe, AZ 85287-1601); Mason; Hugh S. (1311 Hanshaw Rd., Ithaca, NY 14850)

Assignees: Boyce Thompson Institute for Plant Research (Ithaca, NY); Yissum Research Development Co. (Jerusalem, Israel)

Expression of recombinant human acetylcholinesterase in transgenic plants 

Patent No. 6,770,799

Issued: August 3, 2004
Filed: March 16, 2001

Briefly stated, the invention includes a method of making a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase, comprising the steps of introducing into at least one plant cell a polynucleotide that encodes a human acetylcholinesterase, and regenerating from the plant cell a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase in at least one tissue type of the transgenic plant. Another embodiment of the invention includes a method of making a physiologically active human acetylcholinesterase, comprising the steps of introducing into at least one plant cell a polynucleotide that encodes a human acetylcholinesterase, regenerating from the plant cell a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase in at least one tissue type of the transgenic plant, and isolating or purifying from the transgenic plant or a part thereof a physiologically active human acetylcholinesterase.

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