Sialyiation of N-linked glycoproteins in the baculovirus expression vector system
Patent No. 6,472,175
Issued: October 29, 2002
Patent No. 6,261,805
Issued: July 17, 2001
Inventor: H. Alan Wood, Ithaca, NY
The present disclosure utilizes a novel approach to protein preparation in the baculovirus expression vector system (BEVS). Specifically, the present invention analyzes the effects of microgravity/low shear on complex glycosylation of proteins prepared via BEVS, including the addition of terminal sialic acid residues to N-linked oligosaccharides.
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